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Plasma proteome of buffaloes
Author(s) -
Pontes Leticia Gomes,
Cavassan Nayara Rodrigues Vieira,
Barros Luciana Curtolo,
Ferreira Junior Rui Seabra,
Barraviera Benedito,
Santos Lucilene Delazari dos
Publication year - 2017
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201600138
Subject(s) - blood proteins , albumin , fibrinogen , proteome , transthyretin , biochemistry , glycoprotein , retinol binding protein , murrah buffalo , chemistry , biology , vitamin , endocrinology , retinol , agronomy
Abstract The proteomic approach has aroused the interest of veterinary medicine researchers, especially regarding the production of biopharmaceuticals and diagnosis of diseases in farm animals. Water buffaloes have gained prominence in the world economy due to the quality of their milk, meat, and leather, in addition to being an important donor of blood components. This work aimed to identify and characterize the proteins present in the blood plasma of Murrah buffaloes ( Bubalus bubalis ) through 2D electrophoresis, in gel protein digestion followed by mass spectrometry technique and for albumin depletion, in solution protein digestion followed by shotgun analysis. Our results showed the identification of 112 protein spots and 35 individual proteins, respectively. The abundant proteins were represented by albumin, fibrinogen‐α, fibrinogen‐β, fibrinogen‐γ, immunoglobulins in general, α‐1‐antiproteinase, α‐1B‐glycoprotein, α‐2‐HS‐glycoprotein, α‐macroglobulin, apolipoprotein A1, antithrombin‐III, endopin 2B, fetuin‐B, retinol‐binding protein, serotransferrin, transthyretin and vitamin D‐binding protein. Most of these proteins are related to the signaling pathways of the complement system and coagulation cascade. The results allowed a better understanding of the protein composition of these blood components, thus promoting studies on animal health in the search for molecular markers of zoonotic diseases in buffaloes.