Premium
Magnetic‐activated cell sorting (MACS) significantly decreases the hybridization efficiency of fluorescence in situ hybridization (FISH)
Author(s) -
Kuo PaoLin,
Guo HowRan
Publication year - 2001
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.71
Subject(s) - fluorescence in situ hybridization , in situ hybridization , cell sorting , microbiology and biotechnology , in situ , differential centrifugation , biology , fish <actinopterygii> , chromosome , chemistry , flow cytometry , genetics , gene expression , gene , organic chemistry , fishery
Fetal cells were enriched from maternal blood using density gradient centrifugation of Histopaque followed by magnetic‐activated cell sorting (MACS) to select CD71‐positive cells. For each specimen, cells partially purified by Histopaque were split into equal portions, and each portion was subjected to purification by MACS in parallel. Cells before and after MACS were subjected to dual‐color fluorescence in situ hybridization (FISH) analysis with X‐ and Y‐chromosome‐specific probes. We found that the hybridization rates were decreased by approximately 10% after MACS based on duplicated analysis for each sample. Copyright © 2001 John Wiley & Sons, Ltd.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom