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Absence of BRAF mutation in pediatric and adolescent germ cell tumors indicate biological differences to adult tumors
Author(s) -
MasquéSoler Neus,
Szczepanowski Monika,
Leuschner Ivo,
Vokuhl Christian,
Haag Jochen,
Calaminus Gabriele,
Klapper Wolfram
Publication year - 2012
Publication title -
pediatric blood and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.116
H-Index - 105
eISSN - 1545-5017
pISSN - 1545-5009
DOI - 10.1002/pbc.24005
Subject(s) - msh6 , kras , mlh1 , microsatellite instability , medicine , germ cell tumors , cancer research , mutation , germ cell , oncology , gene , dna mismatch repair , genetics , colorectal cancer , biology , cancer , microsatellite , chemotherapy , allele
Abstract The V600E mutation of the BRAF gene has been reported to be associated with poor prognosis of germ cell tumors in adult patients. We analyzed the mutational status of the BRAF and KRAS gene as well as MLH1 and MSH6 expression as surrogate markers for microsatellite instability in 70 pediatric germ cell tumors. Neither BRAF and KRAS mutations nor loss of MLH1 and MSH6 expression were found. Our data provide further evidence for patient age related biological differences in germ cell tumors and demonstrate that prognostic biomarkers cannot necessarily be transferred from one age group to the other. Pediatr Blood Cancer 2012;59:732–735. © 2011 Wiley Periodicals, Inc.