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Nerve growth factor release from the urothelium increases via activation of bladder C‐fiber in rats with cerebral infarction
Author(s) -
Yokokawa Ryusei,
Akino Hironobu,
Ito Hideaki,
Zha Xinmin,
Yokoyama Osamu
Publication year - 2017
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.23142
Subject(s) - resiniferatoxin , urothelium , purinergic receptor , cystometry , medicine , urinary bladder , overactive bladder , receptor , ischemia , cerebral infarction , endocrinology , anesthesia , in vivo , nerve growth factor , pharmacology , pathology , trpv1 , biology , transient receptor potential channel , alternative medicine , microbiology and biotechnology
Aims There are some reports that bladder C‐fibers are partially involved in detrusor overactivity in patients with brain lesions. We investigated the contribution of bladder C‐fiber to decreased bladder capacity in rats with cerebral infarction. Methods Cerebral infarction was induced under halothane anesthesia by left middle cerebral artery occlusion with 4‐0 nylon thread in female Sprague‐Dawley rats. Intramural amounts of ATP and prostaglandin E 2 , in vivo and in vitro ATP, NGF, and prostaglandin E 2 release from the distended bladder urothelium, and changes in mRNA expressions of sensor molecules and receptors were monitored 6 h after the occlusion. Cystometry was performed in rats with or without resiniferatoxin pretreatment. Results Overexpression of sensor molecule, transient receptor potential vanilloid‐type channel 1, acid‐sensing ion channel 2, purinergic receptors P2X 3 , and M 2 /M 3 muscarinic receptors was found in the bladder. These changes were accompanied by increases in ATP and NGF release from the urothelium. In contrast, when bladder C‐fibers were desensitized by resiniferatoxin, no increase in NGF release from the urothelium was found either in vivo or in vitro. There was no difference in the percentage decrease in bladder capacity between cerebral infarction rats pretreated with resiniferatoxin and cerebral infarction rats without pretreatment. Conclusions Results indicate that expression of sensor molecules in the bladder is altered by distant infarction in the brain. ATP and NGF release from the urothelium also increased. NGF release was related to activation of bladder C‐fibers. Bladder C‐fibers might not contribute much to decreased bladder capacity caused by cerebral infarction.