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Effects of supplementation of BH 4 after prolonged ischemia in skeletal muscle
Author(s) -
Wang Wei Z.,
Fang XinHua,
Stephenson Linda L.,
Khiabani Kayvan T.,
Zamboni William A.
Publication year - 2007
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.20331
Subject(s) - cremaster muscle , medicine , skeletal muscle , ischemia , vasodilation , reperfusion injury , arteriole , perfusion , microcirculation , endocrinology , endothelial nos , nitric oxide , nitric oxide synthase , anesthesia , enos
Abstract Purpose: To determine whether the supplementation of tetrahydrobiopterin (BH 4 , an essential cofactor of nitric oxide synthase; NOS) could attenuate endothelial dysfunction and improve NOS activity and cell viability in skeletal muscle after ischemia/reperfusion (I/R). Methods: A vascular pedicle isolated rat cremaster muscle model was used. Cremaster muscles were subjected to 4 h of ischemia followed by 2 h of reperfusion. Rats were given either normal saline or BH 4 by intravenous injection at 1 min prior to reperfusion. After reperfusion, average arteriole diameter, capillary perfusion, endothelial‐dependent/‐independent vasodilatation, NOS activity, and muscle cell viability were evaluated. Results: Supplementation of BH 4 prior to reperfusion significantly attenuated reperfusion‐induced vasoconstriction, poor capillary perfusion, and endothelial dysfunction and enhanced cNOS activity and slightly improved cell viability in the skeletal muscle after I/R. Conclusion: Supplementation of BH 4 during reperfusion provided a significant protection against I/R injury in rat skeletal muscle. © 2007 Wiley‐Liss, Inc. Microsurgery, 2007.