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Mutational analysis of a dominant oncogene (c‐Ki‐ ras ‐2) and a tumor suppressor gene ( p53 ) in hamster lung tumorigenesis
Author(s) -
Oreffo Victor I. C.,
Lin HsiuWei,
Gumerlock Paul H.,
Kraegel Susan A.,
Witschi Hanspeter
Publication year - 1992
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940060305
Subject(s) - biology , carcinogenesis , hamster , oncogene , gene , microbiology and biotechnology , tumor suppressor gene , polymerase chain reaction , cancer research , mutation , oligonucleotide , suppressor , mesocricetus , genetics , cell cycle
Abstract In human lung cancers, alterations of both a dominant oncogene ( ras ) and a tumor suppressor gene ( p53 ) have been identified. Polymerase chain reaction (PCR) analysis of mRNA was used to amplify the c‐Ki‐ ras ‐2 and p53 genes from Syrian golden hamsters. The PCR products were confirmed by predicted‐size analysis, probing with nonradioactive (biotin‐labeled) oligonucleotides, and direct sequencing. Lung tumors were produced in hamsters by repeated injections of 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanone (NNK). Of six tumors examined, three (50%) had mutations in codon 12 of Ki‐ ras . Examination of the conserved regions of p53 revealed no mutations. We conclude that NNK‐induced carcinogenesis in the hamster results in characteristic alterations of Ki‐ ras but may not necessarily involve the p53 gene. © 1992 Wiley‐Liss, Inc.