Premium
Homogeneous Detection of Trypsin in Protein Mixtures Based on Fluorescence Resonance Energy Transfer between Anionic Conjugated Polymer and Fluorescent Probe
Author(s) -
Zhu Qing,
Zhan Ruoyu,
Liu Bin
Publication year - 2010
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200900909
Subject(s) - conjugated system , trypsin , fluorescence , förster resonance energy transfer , polymer , homogeneous , chemistry , materials science , combinatorial chemistry , organic chemistry , optics , enzyme , physics , thermodynamics
Abstract Herein we describe a novel and simple conjugated polymer‐fluorescent probe based platform for trypsin detection from protein mixtures in homogeneous solution. This platform takes advantage of specific interaction between the probe and the active site of trypsin and the electrostatic interaction between the polymer and the protein to mediate energy transfer between the polymer and the probe. This method does not require any separation steps, which should facilitate high‐throughput protease screening and drug discovery.