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In Situ Mineralization of Hydroxyapatite on Electrospun Chitosan‐Based Nanofibrous Scaffolds
Author(s) -
Yang Dongzhi,
Jin Yu,
Zhou Yingshan,
Ma Guiping,
Chen Xiangmei,
Lu Fengmin,
Nie Jun
Publication year - 2008
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200700221
Subject(s) - chitosan , biocomposite , fourier transform infrared spectroscopy , chemical engineering , polyvinyl alcohol , polymer chemistry , acrylic acid , nanofiber , electrospinning , materials science , polyacrylic acid , scanning electron microscope , chemistry , tissue engineering , nuclear chemistry , composite number , polymer , composite material , biomedical engineering , monomer , medicine , engineering
Abstract A biocomposite of hydroxyapatite (HAp) with electrospun nanofibrous scaffolds was prepared by using chitosan/polyvinyl alcohol (CS/PVA) and N ‐carboxyethyl chitosan/PVA (CECS/PVA) electrospun membranes as organic matrix, and HAp was formed in supersaturated CaCl 2 and KH 2 PO 4 solution. The influences of carboxylic acid groups in CECS/PVA fibrous scaffold and polyanionic additive poly(acrylic acid) (PAA) in the incubation solution on the crystal distribution of the HAp were investigated. Field‐emission scanning electron microscopy (FE‐SEM), energy‐dispersive spectroscopy (EDS), wide‐angle X‐ray diffraction (WAXD), and Fourier transform infrared (FTIR) were used to characterize the morphology and structure of the deposited mineral phase on the scaffolds. It was found that addition of PAA to the mineral solution and use of matrix with carboxylic acid groups promoted mineral growth and distribution of HAp. MTT testing and SEM imaging from mouse fibroblast (L929) cell culture revealed the attachment and growth of mouse fibroblast on the surface of biocomposite scaffold, and that the cell morphology and viability were satisfactory for the composite to be used in bioapplications.