Premium
Capillary electrophoretic and mass spectrometric analysis of a polydisperse fluorosurfactant
Author(s) -
AlJarah Suhair Yousif,
Sjödahl Johan,
Woldegiorgis Andreas,
Emmer Åsa
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200400040
Subject(s) - capillary electrophoresis , chromatography , chemistry , capillary electrophoresis–mass spectrometry , micellar electrokinetic chromatography , electrophoresis , mass spectrometry , capillary action , electrokinetic phenomena , vinyl alcohol , electrospray ionization , materials science , polymer , composite material , organic chemistry
Abstract A fluorosurfactant has been studied using capillary electrophoresis and mass spectrometry. The fluorosurfactant, FC134, can be used as a buffer additive in capillary electrophoresis in order to decrease wall adsorption of proteins and in micellar electrokinetic chromatography. However, it has been discovered that this fluorosurfactant is polydisperse, thus containing substances with different lengths and structures. In this work, the fluorosurfactant sample components were separated by capillary electrophoresis. An uncoated as well as a poly(vinyl alcohol)‐coated capillary were used with running electrolytes containing methanol and acetic acid. Following the capillary electrophoretic separation, fractions were collected for further analysis by MALDI‐MS. Non‐fractionated samples were also analyzed both by MALDI‐MS and by ESI‐MS.