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Factors influencing chromatographic data in fast gas chromatography with on‐column injection
Author(s) -
Matisová Eva,
Šimeková Mária,
Hrouzková Svetlana,
Korytár Peter,
Dömötörová Milena
Publication year - 2002
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200290003
Subject(s) - chromatography , volatility (finance) , analyte , analytical chemistry (journal) , chemistry , volume (thermodynamics) , gas chromatography , two dimensional chromatography , inlet , instrumentation (computer programming) , injection port , column (typography) , monolithic hplc column , materials science , high performance liquid chromatography , structural engineering , financial economics , economics , mechanical engineering , physics , quantum mechanics , connection (principal bundle) , computer science , engineering , composite material , operating system
Abstract The use of larger volume injection with on‐column injection and fast GC commercial instrumentation was evaluated with the model mixture of n ‐alkanes of a broad range of volatility (C 10 –C 28 ). The presented configuration allows introduction of 40–80‐fold larger sample volumes without any distortion of peak shapes compared to “usual” fast GC set‐ups using narrow‐bore columns. A normal‐bore retention gap (1–5 m×0.32 mm ID) was coupled to a narrow‐bore (5 m×0.1 mm ID×0.4 μm film thickness) analytical column using a standard press‐fit connector. The connection was tight and reliable, and hence suitable for hydrogen as carrier gas. The effect of pre‐column and analytical column connector, injection volume, pre‐column length, column inlet pressure, and analyte volatility on peak shape, peak broadening, and focusing are discussed. The precision of chromatographic data measurements and peak capacity under optimised temperature programmed conditions for fast separations with large volume injection were found to be very good. The presented fast GC set‐up with on‐column injection extends the applicability of the technique to trace analysis.