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Genetic differentiation between the clam species Ruditapes decussatus (grooved carpet shell) and Venerupis pullastra (pullet carpet shell) by PCR–SSCP analysis
Author(s) -
Fernández Alicia,
García Teresa,
Asensio Luis,
Rodríguez Miguel Ángel,
González Isabel,
Lobo Esther,
Hernández Pablo E,
Martín Rosario
Publication year - 2002
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.1117
Subject(s) - biology , single strand conformation polymorphism , dna , amplicon , polymerase chain reaction , gel electrophoresis , staining , gene , zoology , microbiology and biotechnology , genetics
Abstract Genetic differentiation of the clam species Ruditapes decussatus (grooved carpet shell) and Venerupis pullastra (pullet carpet shell) has been achieved based on polymerase chain reaction–single‐strand conformation polymorphism (PCR–SSCP) analysis. A short fragment (150 bp) of the α‐actin gene was amplified by PCR. Amplicons were denatured to obtain single‐stranded DNA, electrophoresed on a non‐denaturing polyacrylamide gel and visualised by silver staining for detection of SSCPs. Species‐specific DNA band patterns were obtained for R decussatus and V pullastra , allowing clear differentiation of the two clam species. © 2002 Society of Chemical Industry