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Cartilage repair by human umbilical cord blood‐derived mesenchymal stem cells with different hydrogels in a rat model
Author(s) -
Park YongBeom,
Song Minjung,
Lee ChoongHee,
Kim JinA,
Ha ChulWon
Publication year - 2015
Publication title -
journal of orthopaedic research®
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22950
Subject(s) - self healing hydrogels , hyaluronic acid , mesenchymal stem cell , umbilical cord , cartilage , biomedical engineering , in vivo , tissue engineering , chemistry , materials science , medicine , pathology , anatomy , biology , polymer chemistry , microbiology and biotechnology
Abstract This study was carried out to assess the feasibility of human umbilical cord blood‐derived mesenchymal stem cells (hUCB‐MSCs) in articular cartilage repair and to further determine a suitable delivering hydrogel in a rat model. Critical sized full thickness cartilage defects were created. The hUCB‐MSCs and three different hydrogel composites (hydrogel A; 4% hyaluronic acid/30% pluronic (1:1, v/v), hydrogel B; 4% hyaluronic acid, and hydrogel C; 4% hyaluronic acid/30% pluronic/chitosan (1:1:2, v/v)) were implanted into the experimental knee (right knee) and hydrogels without hUCB‐MSCs were implanted into the control knee (left knee). Defects were evaluated after 8 weeks. The hUCB‐MSCs with hydrogels composites resulted in a better repair as seen by gross and histological evaluation compared with hydrogels without hUCB‐MSCs. Among the three different hydrogels, the 4% hyaluronic acid hydrogel composite (hydorgel B) showed the best result in cartilage repair as seen by the histological evaluation compared with the other hydrogel composites (hydrogel A and C). The results of this study suggest that hUCB‐MSCs may be a promising cell source in combination with 4% hyaluronic acid hydrogels in the in vivo repair of cartilage defects. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1580–1586, 2015.