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Hybridization of yeasts by protoplast fusion: Ploidy level of hybrids resulting from fusions in haploid strains of Pichia guilliermondii
Author(s) -
Klinner U.,
Böttcher F.
Publication year - 1984
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19840240806
Subject(s) - ploidy , protoplast , hybrid , biology , auxotrophy , mitosis , cell fusion , mutant , strain (injury) , genetics , botany , cell culture , gene , anatomy
Hybrids produced by protoplast fusion between auxotrophic mutants of the same strain contained the parental markers heterozygously, as indicated by induced mitotic segregation. Even within the same fusion combination the hybrids differed in their mitotic stability and their induced mitotic segregation pattern. The occurrence of aneuploids was proved. The ploidy of the hybrids did not exceed the diploid level in any case.