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Protoplastenfreisetzung und Regeneration des Ascomyceten Hypomyces ochraceus (PERS.) TUL
Author(s) -
Riemay K. H.,
Ellrich Silvia,
Tröger R.
Publication year - 1983
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19830230406
Subject(s) - protoplast , helix pomatia , germ tube , hypha , mycelium , regeneration (biology) , phase contrast microscopy , chemistry , biology , botany , microbiology and biotechnology , ecology , physics , snail , optics
A rapid and convenient method for producing protoplasts from 3 d old mycelium of the ascomycete Hypomyces ochraceus is described. The procedure involves a Helix pomatia enzyme preparation and sucrose (20%) for stabilization. Pretreatment with disulfide bond reducing agents reduced the amount of viable protoplasts. Formation of protoplasts and different stages of regeneration were observed by phase contrast microscopy. There was only one type of true regeneration from protoplasts to hyphae in 15–30% gelatine medium by direct forming a germ tube from the original protoplast. Cytological events and physiological conditions are discussed.