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Gewinnung und Charakterisierung kohlenhydratspezifischer Antikörper aus anti‐Candida utilis Serum
Author(s) -
Rademacher K.H.
Publication year - 1982
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19820220207
Subject(s) - mannan , sepharose , chemistry , chromatography , oligosaccharide , affinity chromatography , serology , antibody , agglutination (biology) , hydrolysis , biochemistry , microbiology and biotechnology , biology , enzyme , immunology , polysaccharide
Mannan of Candida utilis was hydrolyzed in oligosaccharides of different chain lenghts. Mannohexaose and mannopentaose were shown to be the immunodominant group of this mannan. The oligosaccharides obtained were bound to sepharose by way of β‐(p‐aminophenyl) ethylamine. Immunoglobulins against mannohexaose down to mannodiose were obtained using affinity chromatography of the anti‐C. utilis serum. The affinity columns were filled with sepharose‐oligosaccharide gels. The serological activity was proved by agglutination and precipitation.