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Host components required for the replication of the resistance plasmid R124 and a copy mutant derivative
Author(s) -
Pritchard J. J.,
Rowbury R. J.
Publication year - 1980
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19800200208
Subject(s) - dnab helicase , dnaa , plasmid , mutant , dnag , gene , dna replication , biology , microbiology and biotechnology , origin of replication , genetics , eukaryotic dna replication , rna , helicase
The replication of R124, and a copy mutant derivative of it, was measured with respect to dependence on the host DnaA, DnaB, DnaC, DnaE, DnaG, and Po1A gene products. Both plasmids replicated under conditions where the DnaA gene product was inactivated or where the polymerising activity of the PolA gene product was reduced. In contrast, neither plasmid replicated to any appreciable extent, if the DnaB, DnaC, DnaE or DnaG gene products were inactivated. R124 integratively suppressed the lesion of the dnaA mutant but the copy mutant derivative had only a very weak suppressing effect. Neither plasmid suppressed the lesions of any of the other dna mutants.