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Orthophosphorsäuremonoester‐Phosphohydrolaseaktivität der repressiblen Pyrohosphat‐Phosphohydrolase I von Candida utilis
Author(s) -
Jungnickel F.
Publication year - 1972
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19720120603
Subject(s) - derepression , enzyme , pyrophosphate , chemistry , yeast , biochemistry , phosphate , nuclear chemistry , gene expression , psychological repression , gene
Two enzymes splitting inorganic pyrophosphate (I and II) have been investigated in Candida utilis . The results indicate that only enzyme I (no considerable thermoinactivation — 10 min, 71 °C‐, optimum near pH 2.5, enzyme strongly bound to the cell, high splitting rates in vivo , derepression under phosphate starvation conditions) is also able to split orthophosphoric monoesters. Thus, the enzyme I is an unspecific acid orthophosphoric monoester phosphohydrolase (E. C. 3.1.3.2.).