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Double isotopic method using dansyl chloride for the determination of GABA in rat C6 astrocytoma cell cultures
Author(s) -
Kohl R.L.,
Quay W.B.,
PerezPolo J.R.
Publication year - 1986
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490160311
Subject(s) - dansyl chloride , chloride , chemistry , chromatography , selectivity , quantitative analysis (chemistry) , amino acid , derivatization , biochemistry , high performance liquid chromatography , organic chemistry , catalysis
Abstract Methods are described for the quantitative measurement of GABA in culture. The method can be adapted to any amino acid or dansyl‐chloride‐reactive species. The sensitivity and selectivity of the procedure result from the double isotopic design in which ( 14 C)‐labeled internal standard was added to the samples before reaction with ( 3 M)‐labeled dansyl chloride. Values obtained by ion‐exchange amino acid analysis of cultures agree closely with the values obtained by the double isotopic method. This method is sensitive enough to measure GABA intracellularly and the condition medium.