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Ternary complex with Trk, p75, and an ankyrin‐rich membrane spanning protein
Author(s) -
Chang MiSook,
Arevalo Juan Carlos,
Chao Moses V.
Publication year - 2004
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20262
Subject(s) - trk receptor , neurotrophin , tropomyosin receptor kinase a , low affinity nerve growth factor receptor , microbiology and biotechnology , ankyrin , receptor tyrosine kinase , biology , receptor , signal transducing adaptor protein , tropomyosin receptor kinase b , signal transduction , neurotrophic factors , biochemistry , gene
Abstract Neurotrophins play many critical roles in regulating neuronal plasticity, survival, and differentiation in the nervous system. Neurotrophins recognize two different receptors, the Trk receptor tyrosine kinase and the p75 neurotrophin receptor, which are associated closely. Several adaptor proteins are associated with each receptor. An ankyrin‐rich membrane spanning protein (ARMS), originally identified as a substrate for protein kinase D (Kidins220) and as a p75 interacting protein, serves as a novel downstream target of Trk receptor tyrosine kinases. Kidins220/ARMS is co‐expressed frequently with Trk and p75 and represents the only membrane‐associated protein known to interact with both receptors. We report here that a ternary complex can be formed between Trk, p75, and Kidins220/ARMS. The extracellular domains of the TrkA and the p75 receptors are necessary for their association, whereas the juxtamembrane region of p75 was responsible for the interaction with Kidins220/ARMS. Interestingly, increasing the level of Kidins220/ARMS expression resulted in a decreased association of TrkA with p75. These findings thus suggest that Kidins220/ARMS plays an important role in regulating interactions between Trk and p75 neurotrophin receptors. © 2004 Wiley‐Liss, Inc.