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Collision‐induced fragmentation of deprotonated methoxylated flavonoids, obtained by electrospray ionization mass spectrometry
Author(s) -
Justesen Ulla
Publication year - 2001
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.118
Subject(s) - chemistry , fragmentation (computing) , deprotonation , electrospray ionization , mass spectrometry , electrospray , flavonoid , mass spectrum , ion , polyatomic ion , molecule , stereochemistry , chromatography , analytical chemistry (journal) , organic chemistry , antioxidant , computer science , operating system
Abstract Electrospray operated in the negative mode was used to analyse methoxylated flavonoids. They were found to produce radical anions by collision‐induced fragmentation of the aglycones. Loss of a methyl group from the deprotonated molecule corresponding to [M − H − 15] − . ions, as well as [M − H − 15–28] − . and [M − H − 15–29] − fragment ions, were found to constitute the characteristic fragmentation for the monomethoxylated species, whereas [M − H − 15] − . , [M − H − 30] − and [M − H − 30–28] − were predominant for the polymethoxylated species. Obtained under similar conditions, the product‐ion spectra of isomeric compounds were characteristically different. It is therefore possible to distinguish between methoxylated flavonoids with identical molecular mass, e.g. when screening plant extracts for flavonoid composition. However, comparison with standard compounds is necessary for the identification of unknown flavonoid aglycones. Copyright © 2001 John Wiley & Sons, Ltd.