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Synthesis of 14 C‐uridine‐5′ ‐triphosphate by enzymes of escherichia coli cell‐free extracts
Author(s) -
Pumpen P.,
Gren E.
Publication year - 1972
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590080205
Subject(s) - escherichia coli , yield (engineering) , uridine triphosphate , chemistry , enzyme , biochemistry , ribose , uracil , uridine , adenosine triphosphate , phosphate , nucleotide , dna , rna , materials science , metallurgy , gene
Crude cell‐free extracts of Escherichia coli AB 259 Hfr 3000, thi‐perform multistep conversion of 14 C‐uracil to 14 C‐UTP in vitro in the presence of D‐ribose/or ribose‐5‐phosphate/, ATP and ATP‐regenerating system. ATP concentration is crucial for significant 14 C‐UTP production. Quantitative yield of the product combined with relatively simple procedure and inexpensiveness of initial materials makes the method advantageous for large‐scale preparation of 14 C‐UTP.