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The production of 15 N‐labelled S‐adenosylmethionine and adenine by yeast biosynthesis
Author(s) -
Zappia V.,
Salvatore F.,
Zydek C. R.,
Schlenk F.
Publication year - 1968
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590040305
Subject(s) - yeast , biosynthesis , methionine , biochemistry , chemistry , hydrolysis , irradiation , yeast extract , ion exchange , ion chromatography , isolation (microbiology) , biology , fermentation , enzyme , ion , amino acid , organic chemistry , microbiology and biotechnology , physics , nuclear physics
Abstract A method for the biosynthesis of 15 N‐labelled S‐adenosylmethionine from 15 NH 4 + and L‐methionine by yeast is reported. Culture conditions suitable for economy in the utilization of the isotope were developed, and the use of γ‐irradiated yeast (1 Mrad) for this purpose was explored. The irradiated yeast proved as effective as non‐irradiated yeast, but it did not offer any advantage. 15 N‐Adenine was obtained from S‐adenosylmethionine by hydrolysis; its isolation by ion exchange chromatography is described.