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Vérification de la pureté radiochimique d'un isomère optique marqué
Author(s) -
Verly Walter G.,
Gerday Charles
Publication year - 1965
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590010105
Subject(s) - diastereomer , enantiomer , chemistry , dipeptide , phenylalanine , amino acid , enantiomeric excess , chromatography , stereochemistry , catalysis , organic chemistry , biochemistry , enantioselective synthesis
A method for evaluating the radiochemical purity of the labelled isomer of an amino acid is described. It permits to measure the quantity of the enantiomer present as an impurity. The method is applied to the case of tritiated L‐phenylalanine. The inactive racemic form, added as a carrier, is condensed with L‐glutamic acid; the diastereoisomers of the resulting dipeptide are resolved chromatographically on Dowex 50 and the radioactivity of each peak is measured. This process requires only a very little sample and it enables the detection of very minute contaminants.