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Metabolism of [ 11 C‐ methyl ]choline in tumour bearing mice and synthesis and isolation of its catabolite [ 11 C‐ methyl ]betaine
Author(s) -
Brown G. D.,
Osman S.,
Wilson H. K.,
Aboagye E.,
Price P. M.,
Luthra S. K.,
Brady F.
Publication year - 2001
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580440137
Subject(s) - betaine , choline , chemistry , metabolism , phosphocholine , stereochemistry , labelling , biochemistry , methyl group , organic chemistry , phospholipid , membrane , phosphatidylcholine , alkyl
The uptake and metabolism of [ 11 C‐ methyl ]choline was examined in tumour bearing mice. Tumours showed [ 11 C‐ methyl ]choline, [ 11 C‐ methyl ]betaine and [ 11 C‐ methyl ]phosphocholine, whereas in plasma only [ 11 C‐ methyl ]choline and [ 11 C‐ methyl ]betaine were observed. To correct for the contribution of [ 11 C‐ methyl ]betaine to [ 11 C‐ methyl ]choline derived radioactivity in tissues, [ 11 C‐ methyl ]betaine has been prepared from [ 11 C‐ methyl ]choline, isolated by HPLC and the position of incorporation of C‐11 label confirmed by [ 11/13 C]co‐labelling and NMR.