Premium
Synthesis of tritiated tripeptides and their very simple purification procedures
Author(s) -
Angelini G.,
Sparapani C.,
Margonelli A.
Publication year - 1992
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580311003
Subject(s) - chemistry , yield (engineering) , tripeptide , tritiated water , tritium , radiochemistry , chromatography , peptide , biochemistry , materials science , physics , nuclear physics , metallurgy
The complete reaction pathway (five steps) for the syntheses of tritiated [CBZ‐Pro‐ * Leu‐TrpOH] 7 (POL‐443) and [Pyr‐ * Leu‐TrpOMe] 5 (POL‐509) starting from tritiated * Leu‐[3, 4, 5‐ 3 H(N)] is described. The products 5 and 7 are obtained with high level of chemical and radiochemical purity and in good total yield (70% for 5 and 60% for 7) (1). The use of the “Solid Phase Extraction” procedure made the purification and recovery steps very simple and effective with no isotopic exchange.