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Enzymatic synthesis of isotopically labelled pyrimidine deoxyribonucleotides
Author(s) -
Myasoedov N. F.
Publication year - 1991
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580291005
Subject(s) - deoxyribonucleotides , chemistry , pyrimidine , enzyme , ether , ribonucleotide reductase , deoxyribonucleotide , pyrimidine metabolism , biochemistry , organic chemistry , nucleotide , purine , protein subunit , gene
An enzymatic method is described which makes it possible to synthesize labelled pyrimidine deoxyribonucleotides from corresponding labelled ribonucleotides using the RDP‐reductase system of ether‐treated E. coli E125 cells. Ether‐treated cells have been used for the first time for preparative enzymatic synthesis of labelled biologically active compounds.