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Rapid synthesis of 14C forskolin by a route suitable for 11 C labeling
Author(s) -
Sasaki T.,
OgiharaUmeda I,
Nishigori H.,
Ikegami S.,
Senda M.,
Kitani K.,
Ogawa K.,
Nozaki T.
Publication year - 1991
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580290507
Subject(s) - forskolin , chemistry , yield (engineering) , chromatography , high performance liquid chromatography , acetic acid , methanol , biochemistry , organic chemistry , in vitro , materials science , metallurgy
Radiolabeled forskolin was synthesized by acetylation of deacetylforskolin with 14 C‐labeled acetic acid in the presence of dicyclohexylcarbodiimide. Separation of labeled forskolin of greater than 97% radiochemical purity was achieved by HPLC with a reversed‐phase column. The synthesis and purification of labeled forskolin were achieved in less than 30 min and the radiochemical yield of labeled forskolin after HPLC purification was about 30%, These methods are therefore applicable for production of 11 C‐forskolin which may be useful for PET second messenger imaging.