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A tritiated photolabel specific for the active site of monoamine oxidase
Author(s) -
Backwell F. R. C.,
Williams C. H.
Publication year - 1990
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580280408
Subject(s) - chemistry , monoamine oxidase , flavin group , covalent bond , enzyme , stereochemistry , biochemistry , organic chemistry
We report the synthesis of a tritiated inhibitor of monoamine oxidase, containing a photolabile azido group. The compound, N‐(4‐azido‐2‐nitrophenyl)‐N′‐3‐[ 3 H]‐methyl‐N′‐(3‐prop‐1‐ynyl)‐1,3‐diamino propane, is a novel, specific photolabel designed to interact covalently with the flavin of the enzyme in absence of light and to form a second covalent linkage with the polypeptide on photoirradiation. The compound is shown to label a sample of monoamine oxidase isolated from bovine liver, and may be useful in helping to map the active site of the enzyme.