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A one‐pot radiosynthesis of [ 125 I]iodoazido photoaffinity labels
Author(s) -
Wilson Alan A.,
Grigoriadis Dimitri E.,
Dannals Robert F.,
Ravert Hayden T.,
Wagner Henry N.
Publication year - 1989
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580271109
Subject(s) - chemistry , radiosynthesis , photoaffinity labeling , alkyl , chemical synthesis , radiochemistry , high performance liquid chromatography , combinatorial chemistry , chromatography , organic chemistry , biochemistry , binding site , microbiology and biotechnology , in vivo , biology , in vitro
A useful method for preparing radioiodinated photoaffinity labels from alkyl anilines which offers significant advantages over present methods is described. The one‐pot synthesis gives good radiochemical yields (40–64%) of pure, high specific activity (350–1500 mCi/μmol) 125 I labelled iodoaryl azides while minimising manipulation of radioactive materials. Purification of the [ 125 I]iodoazido photoaffinity labels is achieved by high performance liquid chromatography.