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A method for the preparation of labelled gossypol by the incorporation of 14 C acetate
Author(s) -
Guadalupe Rojas M.,
Stipanovic Robert D.,
Williams Howard J.,
Vinson Bradleigh
Publication year - 1989
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580270903
Subject(s) - gossypol , chemistry , seedling , sodium acetate , crystallization , gossypium , nuclear chemistry , organic chemistry , chromatography , botany , biochemistry , biology
Carbon‐14 14 labelled gossypol has been prepared by incorporating 1,2‐ 14 C acetate in the growth medium of cold shocked cotton ( Gossypium arboreum ) seedling roots. Unlabelled gossypol was added during the purification procedure to aid crystallization; thus, from 350 seedling roots, 25 u Ci sodium (1,2‐ 14 C) (45–60 mCi/mmol) acetate and 800 mg of unlabelled gossypol; 350 mg of labelled gossypol (1900 dpm/mg) was isolated.