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The synthesis of tritium‐labelled azidothymidine
Author(s) -
Aggarwal Sunil K.,
Shalinsky David R.,
Agrawal Krishna C.
Publication year - 1988
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580251002
Subject(s) - chemistry , tritium , thymidine , chromatography , high performance liquid chromatography , column chromatography , radiochemistry , biochemistry , dna , physics , nuclear physics
Abstract The synthesis of 3 H‐labelled azidothymidine (AZT) from [methyl‐ 3 H]thymidine is described. Purification of labelled AZT was accomplished by column chromatography. The purity of the labelled compound was determined by high performance liquid chromatography connected with a radioactive flow detector. The labelled AZT was found to be stable at pH ranging from 2.3 to 7.4 at 37°C and at hyperthermic temperature of 42°C.