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Synthese des Isomeres (E) et (Z) de la Phenylhydrazone De L'hexanal (noyau 14 C ‐ U)
Author(s) -
Chopard C.,
Galey J. B.,
Chottard J. C.
Publication year - 1987
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580240309
Subject(s) - chemistry , hexanal , phenylhydrazine , aniline , ring (chemistry) , chromatography , hydrochloride , high performance liquid chromatography , medicinal chemistry , organic chemistry
Synthesis and chromatographic separation of (E) and (Z) isomers of (ring‐U‐ 14 C) hexanal phenylhydrazone 2 : (ring‐U‐ 14 C) phenylhydrazine hydrochloride 1 was synthesized from labelled aniline hydrochloride, and purification was accomplished by reverse phase low pressure liquid chromatrography. The crude free base of 1 was condensed with hexanal to give a mixture of (E) and (Z) isomers of 2 . The conditions for their separation by reverse phase HPLC were optimized on an analytical column and then used for the preparative work which was the most effective and fastest method. Structure and configuration of (E) and (Z) isomers of (ring‐U‐ 14 C) hexanal phenylhydrazone (SA = 13.3 mCi/mmol) were proven by 1 H‐NMR and mass spectroscopy.