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Synthese d'Hyoroxy‐3 Anorostene‐5 One‐17 (DHA) Tritiee a Haute Activite Specifique
Author(s) -
Pichat L.,
Moustier A. M.,
Lellouche J. P.,
Beaucourt J. P.
Publication year - 1986
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580230706
Subject(s) - chemistry , tritium , high performance liquid chromatography , proton nmr , chromatography , nuclear chemistry , organic chemistry , physics , nuclear physics
Tritiated DHA with a high specific activity is necessary for radioimmunoassays. We synthesized DHA labell with tritium in three positions (Specific Activity: 90 Ci/mM). After protection of the 17‐carbonyl group of the Δ 1,4 androstadiene‐3,17 dione, monobromide 3 was obtained. Dehydrobromination and selective reduction with NaBH 4 led to the precursor 5 which was reduced by tritium. After deprotection DHA‐[l,2,15‐ 3 H] waw obtained purified by liquid chromatography and characterized by TLC, 3 H‐NMR. MS and HPLC. Data concerning U.V. spectra NMR and Optical Rotatory Power are given for the main products and for sidereactions compounds.