Premium
Synthesis of 2,5‐dihydroxy[carboxy‐ 14 C]benzoic acid for electrochemical protein modification
Author(s) -
John Varghese,
Vennerstrom Jonathan L.,
Holmes Thomas J.
Publication year - 1986
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580230308
Subject(s) - gentisic acid , chemistry , benzoic acid , salicylic acid , hydroxylation , yield (engineering) , potassium persulfate , nuclear chemistry , organic chemistry , chromatography , biochemistry , enzyme , materials science , metallurgy , polymerization , polymer
Abstract The synthesis of 2,5‐dihydroxy[carboxy‐ 14 C]benzoic acid (gentisic acid) is described. The procedure utilized [carboxy‐ 14 C]salicylic acid as a starting material. Salicylic acid was converted to gentisic acid using potassium persulfate hydroxylation. The mixture of hydroxylation products in this reaction was easily separated by UV‐visualized quartz column chromatography to obtain the desired isomer in high purity (overall yield of 38%). This procedure may be used for micro and semimicro preparation of the title compound.