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Evaluation of an iodination procedure for arg 8 ‐vasopressin
Author(s) -
Ysewijnvan Brussel K. A. R. N.,
van Steirteghem A. C.,
de Leenheer A. P.
Publication year - 1983
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580200113
Subject(s) - chemistry , vasopressin , chloramine t , iodine , yield (engineering) , chloramine , iodine monochloride , halogenation , specific activity , antiserum , radiochemistry , hormone , medicine , endocrinology , nuclear chemistry , chromatography , biochemistry , organic chemistry , antibody , enzyme , chlorine , materials science , immunology , metallurgy , biology
Arg 8 ‐vasopressin (AVP) was labelled with iodine‐125 using chloramine‐T. The radiochemical yield of 125 I‐AVP was 71% at the specific activity (S.A.) of 784 μCi/μg AVP (29 MBq/μg AVP) as estimated by self‐displacement analysis. Immunoreactivity during storage was determined by the percent binding of 125 I‐ AVP with specific antiserum. Results indicate that chloramine‐T allows high iodine incorporation with limited oxidation damage. Tracers produced were found stable for at least 5 weeks.