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Preparation of 2‐[ 125 I] iodohistamine‐labelled Δ 8 ‐tetrahydrocannabinol‐11‐oic acid for use in cannabinoid radioimmunoassay
Author(s) -
Law B,
Mason P A,
Moffat A C,
King L J
Publication year - 1982
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580190804
Subject(s) - radioimmunoassay , chemistry , cannabinoid , antiserum , chromatography , tetrahydrocannabinol , yield (engineering) , biochemistry , antibody , receptor , materials science , metallurgy , immunology , biology
A simple method is described for the preparation of 2‐[ 125 I]iodohistamine‐labelled Δ 8 ‐tetrahydrocannabinol‐11‐oic acid with high specific activity for use in radioimmunoassay. This compound is produced in high yield and shows excellent radiochemical stability when stored at 4°C. The radiolabelled cannabinoid has been shown to bind avidly to four different broadly specific cannabinoid antisera. It is the only [ 125 I] labelled cannabinoid suitable for use in radioimmunoassay with such antisera.