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Synthesis of carboxy‐labelled l‐carnitine
Author(s) -
Goodfellow Donald B.,
Hoppel Charles L.,
Turkaly Julia S.
Publication year - 1982
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580190308
Subject(s) - carnitine , chemistry , hydroxylation , chemical synthesis , stereochemistry , biochemistry , enzyme , in vitro
A method for the production of carboxy‐labelled l‐carnitine is described. The first step is the chemical synthesis of 4‐N‐trimethylammoniobutanoate (butyrobetaine) from the precursors 4‐aminobutanoate and iodomethane. The second step involves the hydroxylation of butyrobetaine to form l‐carnitine using butyrobetaine hydroxylase partially purified from bovine calf liver. The method also can be used to synthesize Me‐labelled and uniformly‐chain‐labelled l‐carnitine.