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Synthesis of [7‐ 3 H]‐3β, 16β‐dihydoxy‐5‐androsten‐17‐one
Author(s) -
Franklin Ronald B.,
McMurtry Randolph J.,
Nelson Sidney D.
Publication year - 1981
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580180812
Subject(s) - chemistry , steroid , tritium , gas chromatography , thin layer chromatography , chromatography , metabolism , steroid metabolism , radiochemistry , stereochemistry , biochemistry , hormone , physics , nuclear physics
Abstract A method has been described for the synthesis of tritiated [7‐ 3 H]‐3β,16β‐dihydroxy‐5‐androsten‐17‐one of high specific activity starting from [7‐ 3 H]‐5‐androsten‐17‐one. The identity of the radioactive steroid was established by thin layer chromato‐graphy, gas chromatography and gas chromatography combined with mass spectroscopy. The radiolabelled steroid may be used to study its disposition and metabolism in experimental animals and also to further investigate the potential role of 3β,16β‐dihydroxy‐5‐androsten‐17‐one in patients with low renin essential hypertension.