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Tritium labelling of morpholine on gamma‐irradiated silica gel
Author(s) -
Crossley P. C.,
Hannan S. F.,
Odell A. L.
Publication year - 1979
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580160616
Subject(s) - labelling , chemistry , morpholine , tritium , silica gel , reagent , radiochemistry , tritium illumination , gamma irradiation , hydrogen , irradiation , organic chemistry , biochemistry , physics , nuclear physics
The use of gamma‐irradiated silica gel to promote exchange between tritium gas and carbon‐bound hydrogen has been previously described. (1,2,3) The suitability of this method for labelling molecules containing labile as well as carbon‐bound hydrogen has not hitherto been reported. The labelling of morpholine to facilitate its use as a weak base indicator as part of a study of intracellular pH (4) afforded an opportunity to test the suitability of gamma‐irradiated silica gel as a labelling reagent.