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Synthesis of stereospecifically labelled [4‐ 3 H]cholesterol
Author(s) -
Lockley W. J. S.,
Rees H. H.,
Goodwin T. W.
Publication year - 1978
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580150148
Subject(s) - chemistry , tritium , cholesterol , specific activity , cholesterol synthesis , combinatorial chemistry , stereochemistry , radiochemistry , chromatography , organic chemistry , biochemistry , enzyme , physics , nuclear physics , reductase
[4α‐ 3 H]Cholesterol was prepared in three steps from 4‐oxo‐cholest‐5‐en‐3β‐yl acetate, which was conveniently prepared from cholesterol. Introduction of the 4α 3 H label utilised NaB 3 H 4 as the source of tritium. Details of this synthesis and also the synthesis of [4β‐ 3 H]cholesterol are given. Procedures for quantitative estimation of the tritium label at the 4α and 4β positions are also described.