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Preparation of 35 S‐labelled l‐methionine and l‐cystine by biosynthesis
Author(s) -
Albahari S. R.,
SkakunTodorovi M. B.
Publication year - 1978
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580140510
Subject(s) - methionine , chemistry , cystine , yeast , biosynthesis , hydrolysis , saccharomyces cerevisiae , amino acid , ion chromatography , biochemistry , chromatography , cysteine , enzyme
The modified method for biosynthesis of L‐methionine‐ 35 S and L‐cystine‐ 35 S from baker's yeast ( Saccharomyces cerevisiae ) is described. The yeast was cultivated in a sulphur‐deplated medium containing carrier‐free Na 2 35 SO 4 , under air bubbling during 24 hours. Yeast proteins were hydrolyzed enzymatically into free amino acids, and L‐methionine‐ 35 S and L‐cystine‐ 35 S were isolated by ion‐exchange chromatography on a column. Radioactive yields for L‐methionine‐ 35 S and L‐cystine‐ 35 S were 30% and 10%, respectively. The obtained products have high specific activity, 1–10 Ci/mmol, and radiochemical purity better than 95%.