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An enzymatic procedure for the synthesis of 3 H‐thymidine‐5′‐triphosphate
Author(s) -
Preussel Brunhild,
Fritz Marion,
Lange Peter
Publication year - 1978
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580140418
Subject(s) - chemistry , thymidine , tritium , phosphotransferase , thymidine kinase , enzyme , nucleoside triphosphate , yield (engineering) , nucleoside , radiochemistry , specific activity , ion exchange , ion chromatography , thymidylate synthase , adenosine triphosphate , biochemistry , chromatography , nucleotide , organic chemistry , ion , dna , virus , materials science , virology , herpes simplex virus , biology , nuclear physics , metallurgy , physics , gene , fluorouracil , medicine , surgery , chemotherapy
Abstract An enzymatic method for synthesis of tritium‐labelled thymidine‐5′‐triphosphate is described. The procedure utilizes a nucleoside phosphotransferase from wheat germ and a partially purified thymidylate kinase from calf thymus. The product is isolated by ion‐exchange chromatography on DEAE‐cellulose and can be obtained in a yield of approximately 40% and at a radiochemical purity of 95%.