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A BAC library of the East African haplochromine cichlid fish Astatotilapia burtoni
Author(s) -
Lang Michael,
Miyake Tsutomu,
Braasch Ingo,
Tinnemore Deborah,
Siegel Nicol,
Salzburger Walter,
Amemiya Chris T.,
Meyer Axel
Publication year - 2005
Publication title -
journal of experimental zoology part b: molecular and developmental evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 63
eISSN - 1552-5015
pISSN - 1552-5007
DOI - 10.1002/jez.b.21068
Subject(s) - cichlid , biology , fish <actinopterygii> , genome , zoology , evolutionary biology , fishery , genetics , gene
Abstract A BAC library was constructed from Astatotilapia burtoni , a haplochromine cichlid that is found in Lake Tanganyika, East Africa, and its surrounding rivers. The library was generated from genomic DNA of blood cells and comprises 96,768 individual clones. Its median insert size is 150 kb and the coverage is expected to represent about 14 genome equivalents. The coverage evaluation was based on genome size estimates that were obtained by flow cytometry. In addition, hybridization screens with five probes largely corroborate the above coverage estimate, although the number of clones ranged from 5 to 22 authenticated clones per single copy probe. The BAC library described here is expected to be useful to the scientific community interested in cichlid genomics as an important resource to gain new insights into the rapid evolution of the great species diversity of haplochromine cichlid fishes. J. Exp. Zool. (Mol. Dev. Evol.) 306B, 2006 . © 2005 Wiley‐Liss, Inc.