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Amino acid restriction increases β‐cell death under challenging conditions
Author(s) -
Mateus Gonçalves Luciana,
Vettorazzi Jean Franciesco,
Vanzela Emerielle Cristine,
Figueiredo Mariana Sarto,
Batista Thiago Martins,
Zoppi Claudio Cesar,
Boschero Antonio Carlos,
Carneiro Everardo Magalhães
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28389
Subject(s) - amino acid , programmed cell death , chemistry , microbiology and biotechnology , biology , biochemistry , apoptosis
Abstract Malnutrition programs metabolism, favor dysfunction of β cells. We aimed to establish an in vitro protocol of malnutrition, assessing the effect of amino acid restriction upon the β cells. Insulin‐producing cells INS‐1E and pancreatic islets were maintained in RPMI 1640 medium containing 1× (Ctl) or 0.25× (AaR) of amino acids. We evaluated several markers of β‐cell function and viability. AaR Insulin secretion was reduced, whereas cell viability was unaltered. Calcium oscillations in response to glucose increased in AaR. AaR showed lower Ins1 RNAm, snap 25, and PKC (protein kinase C) protein content, whereas phospho‐eIF2α was increased. AaR cells exposed to nutrient or chemical challenges displayed higher apoptosis rates. We showed that amino acid restriction programmed β cell and induced functional changes. This model might be useful for the study of molecular mechanisms involved with β‐cell programming helping to establish novel therapeutic targets to prevent harmful outcomes of malnutrition.