Premium
Norepinephrine decreases EGF binding in primary rat hepatocyte cultures
Author(s) -
Cruise Jennifer L.,
Cotecchia Susanna,
Michalopoulos George
Publication year - 1986
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041270106
Subject(s) - hepatocyte , epidermal growth factor , incubation , receptor , medicine , dna synthesis , endocrinology , antagonist , norepinephrine , receptor antagonist , biology , adrenergic receptor , chemistry , microbiology and biotechnology , in vitro , biochemistry , dopamine
Abstract Norepinephrine (NE) produced a dose‐dependent inhibition of 125 I‐epidermal growth factor (EGF) binding to adult rat hepatocytes in primary culture. This effect was maximal after 1 hr of incubation with NE and could be blocked by the presence of an α 1 ‐specific adrenergic receptor antagonist. The inhibition of binding correlates with the ability of NE to enhance hepatocyte DNA synthesis in the presence of EGF and appears to be mediated by a reduction in EGF receptor number, without a significant change in receptor affinity.