Open AccessMethod of stabilizing blood for the determination of methemoglobin
Author(s) -
Shihana Fathima,
Dawson Andrew H.,
Dissanayake Dhammika M.
Publication year - 2011
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20487
Subject(s) - methemoglobin , chemistry , phosphate buffered saline , hemoglobin , chromatography , buffer solution , ionic bonding , ionic strength , phosphate , biochemistry , ion , organic chemistry , aqueous solution
Abstract Methemoglobin (MetHb) is a significant clinical problem for some poisonings. Its measurement is a problem as both formation and reduction of MetHb can occur even after sampling with time. The objective of this study was to discover a method to stabilize the blood samples for the determination of MetHb. First, hemolysates were prepared by diluting the MetHb blood samples with phosphate buffers under different pH values. The samples were stored at 4–8°C and a day‐to‐day variability in the amount of MetHb was determined using the method described by Evelyn and Malloy. The results show that there is a significantchange in the amount of MetHbstored in both KH 2 PO 4 /Na 2 HPO 4 and KH 2 PO 4 /Na 2 HPO 4 .2H 2 O buffer solutions at pH of 6.7 and 6.9. Buffer solution containing phosphate composition of KH 2 PO 4 /Na 2 HPO 4 ·2H 2 O (pH=7.0) gives relatively stable values for MetHb during the storage and the amount of MetHb samples in the buffer solution retain constant up to 9 days. Therefore, stabilized MetHb blood samples can be prepared using KH 2 PO 4 /Na 2 HPO 4 ·2H 2 O buffer solution (pH=7) with non‐ionic detergent and the samples can be stored for several days at 4–8°C. J. Clin. Lab. Anal. 25:366–368, 2011. © 2011 Wiley‐Liss, Inc.