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LncRNA CCAT1 promotes autophagy via regulating ATG7 by sponging miR‐181 in hepatocellular carcinoma
Author(s) -
Guo Jianbo,
Ma Yingbo,
Peng Xueqiang,
Jin Hongyuan,
Liu Jingang
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29064
Subject(s) - autophagy , downregulation and upregulation , long non coding rna , cancer research , microrna , hepatocellular carcinoma , cell growth , cell , pathogenesis , chemistry , biology , apoptosis , medicine , gene , biochemistry
Abstract Background Hepatocellular carcinoma (HCC) is a significant clinical challenge, and the mechanisms underlying HCC pathogenesis remain incompletely understood. Colon cancer associated transcript 1 (CCAT1), is one novel long noncoding RNA (lncRNA) which is upregulated in HCC. Autophagy is a vital process in HCC progression, and it is unknown whether CCAT1 regulates autophagy in HCC. Materials and methods Immunofluorescence staining and transmission electron microscopy were used to analyze autophagy activity. Luciferase assay was performed to confirm miRNA‐181a‐5p (miR‐181a‐5p) bind CCAT1 and ATG7. Results CCAT1 levels were higher in tissue and cell lines of HCC. In function research, we found that CCAT1 facilitates HCC cell autophagy and cell proliferation. Our results show that, mechanistically, CCAT1 promotes autophagy through functioning as a sponge for miR‐181a‐5p, and then regulating ATG7 expression. Conclusion Our findings indicate CCAT1 may play a role in regulating autophagy by sponging miR‐181a‐5p in HCC.