Premium
Osteogenic apatite particles by sol–gel assisted electrospraying
Author(s) -
Chakrapani Venkatesan Yogeshwar,
Sampath Kumar T. S.,
Raj Deepa K.,
Kumary T. V.
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34013
Subject(s) - osteocalcin , apatite , alkaline phosphatase , osteopontin , materials science , osteoblast , particle size , nanoparticle , ceramic , chemical engineering , calcium , mesenchymal stem cell , mineralogy , nanotechnology , chemistry , composite material , in vitro , biochemistry , enzyme , metallurgy , immunology , microbiology and biotechnology , engineering , biology
Abstract Electrospraying has tremendous potential to prepare submicron to nano size ceramic particles with novel properties. In this study, a sol–gel assisted electrospraying has been used to synthesise phase controlled apatite (hydroxyapatite, HA and calcium deficient hydroxyapatite, CDHA) particles. Variation in particle size was also achieved by controlling the process parameters. The particles were non cytotoxic, induced proliferation of osteoblast‐like cells (HOS) and internalised by the cells. Increased alkaline phosphatase, collagen and calcium deposition confirmed the mineralisation of cells. Expression of osteopontin, osteocalcin and alkaline phosphatase genes further ascertained that the particles promoted osteogenic commitment of the rat bone marrow‐derived mesenchymal stem cells (rBMSCs). The particles also showed better loading and release of tetracycline drug than accelerated microwave synthesised apatite particles. The methodology for synthesis of ceramic particles may have avenues for a wide range of biomedical applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1941–1954, 2018.