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High molecular weight blood group A trisaccharide‐polyacrylamide glycoconjugates as synthetic blood group A antigens for anti‐A antibody removal devices
Author(s) -
Alikhani Azadeh,
Korchagina Elena Y.,
Chinarev Alexander A.,
Bovin Nicolai V.,
Federspiel William J.
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31466
Subject(s) - immunoadsorption , glycoconjugate , chemistry , conjugate , antibody , streptavidin , trisaccharide , antigen , polyacrylamide , biochemistry , biotin , polyacrylamide gel electrophoresis , biotinylation , avidin , chromatography , biology , immunology , polymer chemistry , mathematical analysis , mathematics , enzyme
Abstract Specific immunoadsorption of blood group antibodies by synthetic antigens immobilized on support matrices in the peri‐transplantation period provides a promising solution to hyperacute rejection risk following ABO‐incompatible transplantation. In this study, we investigated binding interactions between anti‐A antibodies and synthetic blood group A trisaccharide conjugated with polyacrylamide of different molecular weights (30 and 1000 kDa). The glycopolymers were equipped with biotin tags and deposited on streptavidin‐coated sensor chips. The affinity and kinetics of anti‐A antibodies binding to glycoconjugates were studied using surface plasmon resonance (SPR). The high molecular weight conjugate (Atri‐PAA 1000 ‐biotin) enhanced antibody binding capacity by two to three fold compared with the low molecular weight conjugate (Atri‐PAA 30 ‐biotin), whereas varying the carbohydrate content in Atri‐PAA 1000 ‐biotin (20 mol % or 50 mol %) did not affect antibody binding capacity of the glycoconjugate. The obtained results suggest that immunoadsorption devices, especially hollow fiber‐based antibody filters which are limited in available surface area for antigen immobilization, may greatly benefit from the new synthetic high molecular weight polyacrylamide glycoconjugates. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009