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Coating and release of an anti‐inflammatory hormone from PLGA microspheres for tissue engineering
Author(s) -
Go Dewi P.,
Palmer Jason A.,
Gras Sally L.,
O'Connor Andrea J.
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33299
Subject(s) - plga , materials science , tissue engineering , in vivo , biomedical engineering , tumor necrosis factor alpha , protein adsorption , cytokine , inflammation , biodegradable polymer , adsorption , peptide , biophysics , biochemistry , nanotechnology , medicine , chemistry , polymer , nanoparticle , biology , organic chemistry , composite material , microbiology and biotechnology
Abstract Many biomaterials used in tissue engineering cause a foreign body response in vivo, which left untreated can severely reduce the effectiveness of tissue regeneration. In this study, an anti‐inflammatory hormone α‐melanocyte stimulating hormone (α‐MSH) was physically adsorbed to the surface of biodegradable poly (lactic‐ co ‐glycolic) acid (PLGA) microspheres to reduce inflammatory responses to this material. The stability and adsorption isotherm of peptide binding were characterized. The peptide secondary structure was not perturbed by the adsorption and subsequent desorption process. The α‐MSH payload was released over 72 h and reduced the expression of the inflammatory cytokine, Tumor necrosis factor‐α (TNF‐α) in lipopolysaccharide activated RAW 264.7 macrophage cells, indicating that the biological activity of α‐MSH was preserved. α‐MSH coated PLGA microspheres also appeared to reduce the influx of inflammatory cells in a subcutaneous implantation model in rats. This study demonstrates the potential of α‐MSH coatings for anti‐inflammatory delivery and this approach may be applied to other tissue engineering applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.